Subcellular distribution of epothilones in human tumor cells

Subcellular distribution of epothilones in human tumor cells.

Epothilones are a new class of natural and potent antineoplastic agents that stabilize microtubules. Although 12,13-epoxide derivatives are potent antiproliferative agents, the activities of the corresponding 12,13-olefin analogs are significantly decreased. These data were confirmed for two new analogs, 6-propyl-EpoB (pEB) and 6-propyl-EpoD (pED), in comparison with the natural compounds EpoB/...

Subcellular distribution of plasminogen activator in cultured human fibrosarcoma cells.

The subcellular distribution of plasminogen activator (PA) was studied in the human fibrosarcoma cell line HT 1080. The cells were homogenized and the cytoplasmic extract (postnuclear supernatant) was fractionated by rate sedimentation and isopyknic equilibration in continuous sucrose density gra dients. The distribution of PA was compared with that of a number of subcellular marker enzymes. PA...

Subcellular Distribution of Human Leukocytic Cathepsins

I N STUDYING certain properties of peripheral leukocytic cathepsins in health and disease,1’2 we found that whole fresh cells contained two pH optima, 3.5 and 8.5, when denatured hemoglobin was used as a substrate.3 Mounter and Atiyeh,4 employing casein as a substrate for extracts of leukocytes, noted optima at pH 3.0, 5.5, and 8.0. Haschen and Krug,5’#{176} using denatured hemoglobin as substr...

Subcellular distribution of human leukocytic cathepsins.

Subcellular distribution of inorganic and methylated arsenic compounds in human urothelial cells and human hepatocytes.

Epidemiological studies have indicated that exposure of humans to inorganic arsenic in drinking water is associated with the occurrence of bladder cancer. The mechanisms by which arsenic induces this malignancy are still uncertain; however, arsenic metabolites are suspected to play a pivotal role. The aim of the present study was the investigation of uptake capabilities of human urothelial cell...